ACQUITY UPLC色譜柱176001043液相色譜耗材美國沃特世Waters
UPLC方法開發(fā)包—ACQUITY UPLC色譜柱多種選擇性
除了使用小的、亞二微米顆粒技術(shù)，分離度還能通過使用選擇性不同的固定相而得以改變與改善。結(jié)合使用超高效顆粒技術(shù)、低譜帶展寬系統(tǒng)以及不同的色譜柱鍵合相，色譜工作者就可以高效和有效的開發(fā)出耐用的分析方法，大大快于以往。
UPLC方法轉(zhuǎn)換包—在LC技術(shù)平臺間輕松轉(zhuǎn)換
越來越多的公司已經(jīng)意識到UPLC技術(shù)所帶來的益處，包括提高生產(chǎn)率和數(shù)據(jù)質(zhì)量、節(jié)省每個樣品的分析成本、加快產(chǎn)品上市時間等等。ACQUITY UPLC H-Class系統(tǒng)正是這種轉(zhuǎn)換的范本，其在LC平臺轉(zhuǎn)換的同時能夠確保分析方法的有效性。除了方法中的所有參數(shù)根據(jù)色譜柱規(guī)格及顆粒大小進(jìn)行調(diào)整外，成功的分析方法轉(zhuǎn)換需要在不同粒徑的色譜柱之間，保持同樣的分離選擇性和分離度。沃特世公司行業(yè)內(nèi)**的制造工藝不僅保證了不同批次之間**的重現(xiàn)性，也保持了HPLC和UPLC顆粒間的選擇性一致。新的方法轉(zhuǎn)換工具能夠確保色譜分離在HPLC和UPLC之間的成功轉(zhuǎn)換。
UPLC方法驗證包—確保方法耐用性
為了確保分析方法持續(xù)可靠且具有重現(xiàn)性，采用多批色譜填料進(jìn)行檢測和驗證變得尤為重要。作為硅膠雜化顆粒的主要生產(chǎn)商，沃特世公司為不同批次之間的重現(xiàn)性設(shè)立了行業(yè)的基準(zhǔn)。基于信譽**的顆粒和色譜柱生產(chǎn)的過程控制，使批與批和柱與柱之間的變異性*小化，從而為分析方法長期的可靠性提供信心。
ACQUITY UPLC方法開發(fā)套裝[MDK: Method Development Kit]
套裝名稱 數(shù)量 固定相 顆粒大小 配置 部件編號
選擇性*大化UPLC方法開發(fā)套裝：*寬泛的選擇性用于方法開發(fā)，包括在低pH和高pH下。是低離子強度添加劑條件時(例如甲酸)的*佳選擇
選擇性*大化UPLC方法開發(fā)套裝4件/包CSH C18, CSH苯己基, CSH氟苯基, HSS C18 SB CSH 1.7 μm; HSS 1.8 μm 2.1 x 50 mm 176002123
選擇性*大化UPLC方法開發(fā)套裝4件/包CSH C18, CSH苯己基, CSH氟苯基, HSS C18 SB CSH 1.7 μm; HSS 1.8 μm 2.1 x 100 mm 176002124
選擇性*大化UPLC方法開發(fā)套裝4件/包CSH C18, CSH苯己基, CSH氟苯基, HSS C18 SB CSH 1.7 μm; HSS 1.8 μm 3.0 x 50 mm 176002125
選擇性*大化UPLC方法開發(fā)套裝4件/包CSH C18, CSH苯己基, CSH氟苯基, HSS C18 SB CSH 1.7 μm; HSS 1.8 μm 3.0 x 100 mm 176002126
高&低pH，*寬選擇性的UPLC色譜柱套裝：通過開拓低和高流動相pH使分離選擇性*寬
高&低pH，*寬選擇性的UPLC色譜柱套裝4件/包BEH C18, BEH C8, BEH Shield RP18, BEH苯基BEH 1.7 μm 2.1 x 50 mm 176001042
高&低pH，*寬選擇性的UPLC色譜柱套裝4件/包BEH C18, BEH C8, BEH Shield RP18, BEH苯基BEH 1.7 μm 2.1 x 100 mm 176001043
高&低pH，*寬選擇性的UPLC色譜柱套裝4件/包BEH C18, BEH C8, BEH Shield RP18, BEH苯基BEH 1.7 μm 3.0 x 50 mm 176001881
高&低pH，*寬選擇性的UPLC色譜柱套裝4件/包BEH C18, BEH C8, BEH Shield RP18, BEH苯基BEH 1.7 μm 3.0 x 100 mm 176001882
UPLC方法開發(fā)套裝：通過開拓低和高流動相pH(BEH色譜柱)將分離選擇性*大化和適用于極性化合物保留性(HSS T3)
UPLC方法開發(fā)套裝4件/包BEH C18, BEH Shield RP18, BEH 苯基, HSS T3 BEH 1.7 μm; HSS 1.8 μm 2.1 x 50 mm 176001603
UPLC方法開發(fā)套裝4件/包BEH C18, BEH Shield RP18, BEH 苯基, HSS T3 BEH 1.7 μm; HSS 1.8 μm 2.1 x 100 mm 176001604
UPLC方法開發(fā)套裝4件/包BEH C18, BEH Shield RP18, BEH 苯基, HSS T3 BEH 1.7 μm; HSS 1.8 μm 3.0 x 50 mm 176001883
UPLC方法開發(fā)套裝4件/包BEH C18, BEH Shield RP18, BEH 苯基, HSS T3 BEH 1.7 μm; HSS 1.8 μm 3.0 x 100 mm 176001884
L1 UPLC色譜柱套裝：依從USP藥典中L1類別，但提供不同硅醇基活性和疏水性的C18色譜柱
L1 UPLC色譜柱套裝4件/包BEH C18, BEH Shield RP18, HSS C18, HSS T3 BEH 1.7 μm; HSS 1.8 μm 2.1 x 50 mm 176001605
L1 UPLC色譜柱套裝4件/包BEH C18, BEH Shield RP18, HSS C18, HSS T3 BEH 1.7 μm; HSS 1.8 μm 2.1 x 100 mm 176001606
L1 UPLC色譜柱套裝4件/包BEH C18, BEH Shield RP18, HSS C18, HSS T3 BEH 1.7 μm; HSS 1.8 μm 3.0 x 50 mm 176001885
L1 UPLC色譜柱套裝4件/包BEH C18, BEH Shield RP18, HSS C18, HSS T3 BEH 1.7 μm; HSS 1.8 μm 3.0 x 100 mm 176001886
質(zhì)譜UPLC色譜柱套裝：直鏈烷烴C18柱，具有不同的硅醇活性、峰形、選擇性和疏水性，且無MS流失問題
質(zhì)譜UPLC色譜柱套裝4件/包BEH C18, HSS C18, HSS T3, HSS C18 SB BEH 1.7 μm; HSS 1.8 μm 2.1 x 50 mm 176001607
質(zhì)譜UPLC色譜柱套裝4件/包BEH C18, HSS C18, HSS T3, HSS C18 SB BEH 1.7 μm; HSS 1.8 μm 2.1 x 100 mm 176001608
質(zhì)譜UPLC色譜柱套裝4件/包BEH C18, HSS C18, HSS T3, HSS C18 SB BEH 1.7 μm; HSS 1.8 μm 3.0 x 50 mm 176001887
質(zhì)譜UPLC色譜柱套裝4件/包BEH C18, HSS C18, HSS T3, HSS C18 SB BEH 1.7 μm; HSS 1.8 μm 3.0 x 100 mm 176001888
低pH，*寬選擇性UPLC色譜柱套裝：有各種不同的鍵合相和選擇性，用于開發(fā)低pH流動相條件下的反相方法
低pH，*寬選擇性UPLC色譜柱套裝4件/包BEH Shield RP18, BEH 苯基, HSS C18, HSS C18 SB BEH 1.7 μm; HSS 1.8 μm 2.1 x 50 mm 176001609
低pH，*寬選擇性UPLC色譜柱套裝4件/包BEH Shield RP18, BEH 苯基, HSS C18, HSS C18 SB BEH 1.7 μm; HSS 1.8 μm 2.1 x 100 mm 176001610
低pH，*寬選擇性UPLC色譜柱套裝4件/包BEH Shield RP18, BEH 苯基, HSS C18, HSS C18 SB BEH 1.7 μm; HSS 1.8 μm 3.0 x 50 mm 176001889
低pH，*寬選擇性UPLC色譜柱套裝4件/包BEH Shield RP18, BEH 苯基, HSS C18, HSS C18 SB BEH 1.7 μm; HSS 1.8 μm 3.0 x 100 mm 176001890
選擇性*大化的RP 和 HILIC UPLC方法開發(fā)套裝：通過綜合HILIC和RP固定相以提供*寬分離選擇性，用以保留分離跨越寬泛極性范圍的分析物
選擇性*大化的RP 和 HILIC UPLC方法開發(fā)套裝4件/包CSH C18, CSH苯己基, CSH氟苯基, BEH Amide CSH 1.7 μm; BEH 1.7 μm 2.1 x 50 mm 176002127
選擇性*大化的RP 和 HILIC UPLC方法開發(fā)套裝4件/包CSH C18, CSH苯己基, CSH氟苯基, BEH Amide CSH 1.7 μm; BEH 1.7 μm 2.1 x 100 mm 176002128
選擇性*大化的RP 和 HILIC UPLC方法開發(fā)套裝4件/包CSH C18, CSH苯己基, CSH氟苯基, BEH Amide CSH 1.7 μm; BEH 1.7 μm 3.0 x 50 mm 176002129
選擇性*大化的RP 和 HILIC UPLC方法開發(fā)套裝4件/包CSH C18, CSH苯己基, CSH氟苯基, BEH Amide CSH 1.7 μm; BEH 1.7 μm 3.0 x 100 mm 176002130
UPLC RP和HILIC方法開發(fā)套裝：通過綜合RP和HILIC固定相以提供寬分離選擇性，用以保留分離跨越寬泛極性范圍的分析物
UPLC RP和HILIC 方法開發(fā)套裝4件/包BEH C18, BEH Shield RP18, BEH Amide, HSS C18 SB BEH 1.7 μm; HSS 1.8 μm 2.1 x 50 mm 176001959
UPLC RP和HILIC 方法開發(fā)套裝4件/包BEH C18, BEH Shield RP18, BEH Amide, HSS C18 SB BEH 1.7 μm; HSS 1.8 μm 2.1 x 100 mm 176001960
UPLC RP和HILIC 方法開發(fā)套裝4件/包BEH C18, BEH Shield RP18, BEH Amide, HSS C18 SB BEH 1.7 μm; HSS 1.8 μm 3.0 x 50 mm 176001961
UPLC RP和HILIC 方法開發(fā)套裝4件/包BEH C18, BEH Shield RP18, BEH Amide, HSS C18 SB BEH 1.7 μm; HSS 1.8 μm 3.0 x 100 mm 176001962
UPLCHILIC方法開發(fā)套裝：在低pH(對堿性分析物)和高pH(對酸性分析物)下為極性和/或可離子化化合物輕松開發(fā)HILIC方法
UPLCHILIC方法開發(fā)套裝4件/包BEH Amide, BEH HILIC BEH 1.7 μm 2.1 x 50 mm 176001963
UPLCHILIC方法開發(fā)套裝4件/包BEH Amide, BEH HILIC BEH 1.7 μm 2.1 x 100 mm 176001964
UPLCHILIC方法開發(fā)套裝4件/包BEH Amide, BEH HILIC BEH 1.7 μm 3.0 x 50 mm 176001965
UPLCHILIC方法開發(fā)套裝4件/包BEH Amide, BEH HILIC BEH 1.7 μm 3.0 x 100 mm 176001966
ACQUITY UPLC方法轉(zhuǎn)換套裝[MTK: Method Transfer Kit]*
套裝名稱UPLC Column（2.1 mm ID） HPLC Column（4.6 mm ID） 部件編號
CSH C18 1.7 to 5 μm MTK 50 mm, 1.7 μm 150 mm, 5 μm 186005529
CSH Phenyl-Hexyl 1.7 to 5 μm MTK 50 mm, 1.7 μm 150 mm, 5 μm 186005530
CSH Fluoro-Phenyl 1.7 to 5 μm MTK 50 mm, 1.7 μm 150 mm, 5 μm 186005531
BEH C18 1.7 to 5 μm MTK 50 mm, 1.7 μm 150 mm, 5 μm 186004958
BEH Shield RP18 1.7 to 5 μm MTK 50 mm, 1.7 μm 150 mm, 5 μm 186004959
BEH HILIC 1.7 to 5 μm MTK 50 mm, 1.7 μm 150 mm, 5 μm 186004960
HSS C18 1.8 to 5 μm MTK 50 mm, 1.8 μm 150 mm, 5 μm 186004961
HSS T3 1.8 to 5 μm MTK 50 mm, 1.8 μm 150 mm, 5 μm 186004962
HSS C18 SB 1.8 to 5 μm MTK 50 mm, 1.8 μm 150 mm, 5 μm 186004963
HSS Cyano 1.8 to 5 μm MTK 50 mm, 1.8 μm 150 mm, 5 μm 186005979
HSS PFP 1.8 to 5 μm MTK 50 mm, 1.8 μm 150 mm, 5 μm 186006000
CSH C18 1.7 to 3.5 μm MTK 50 mm, 1.7 μm 100 mm, 3.5 μm 186005532
CSH Phenyl-Hexyl 1.7 to 3.5 μm MTK 50 mm, 1.7 μm 100 mm, 3.5 μm 186005533
CSH Fluoro-Phenyl 1.7 to 3.5 μm MTK 50 mm, 1.7 μm 100 mm, 3.5 μm 186005534
BEH C18 1.7 to 3.5 μm MTK 50 mm, 1.7 μm 100 mm, 3.5 μm 186004964
BEH Shield RP18 1.7 to 3.5 μm MTK 50 mm, 1.7 μm 100 mm, 3.5 μm 186004965
BEH HILIC 1.7 to 3.5 μm MTK 50 mm, 1.7 μm 100 mm, 3.5 μm 186004966
BEH Amide 1.7 to 3.5 μm MTK 50 mm, 1.7 μm 100 mm, 3.5 μm 186004967
HSS C18 1.8 to 3.5 μm MTK 50 mm, 1.8 μm 100 mm, 3.5 μm 186004968
HSS T3 1.8 to 3.5 μm MTK 50 mm, 1.8 μm 100 mm, 3.5 μm 186004969
HSS C18 SB 1.8 to 3.5 μm MTK 50 mm, 1.8 μm 100 mm, 3.5 μm 186004970
HSS Cyano 1.8 to 3.5 μm MTK 50 mm, 1.8 μm 100 mm, 3.5 μm 186005980
HSS PFP 1.8 to 3.5 μm MTK 50 mm, 1.8 μm 100 mm, 3.5 μm 186006001
CSH C18 1.7 to 3.5 μm High Rs MTK 100 mm, 1.7 μm 150 mm, 3.5 μm 186005535
CSH Phenyl-Hexyl 1.7 to 3.5 μm
高分辨 MTK 100 mm, 1.7 μm 150 mm, 3.5 μm 186005536
CSH Fluoro-Phenyl 1.7 to 3.5 μm
高分辨 MTK 100 mm, 1.7 μm 150 mm, 3.5 μm 186005537
BEH C18 1.7 to 3.5 μm 高分辨 MTK 100 mm, 1.7 μm 150 mm, 3.5 μm 186004971
BEH Shield RP18 1.7 to 3.5 μm
高分辨 MTK 100 mm, 1.7 μm 150 mm, 3.5 μm 186004972
BEH HILIC 1.7 to 3.5 μm 高分辨 MTK 100 mm, 1.7 μm 150 mm, 3.5 μm 186004973
BEH Amide 1.7 to 3.5 μm 高分辨 MTK 100 mm, 1.7 μm 150 mm, 3.5 μm 186004974
HSS C18 1.8 to 3.5 μm 高分辨 MTK 100 mm, 1.7 μm 150 mm, 3.5 μm 186004975
HSS T3 1.8 to 3.5 μm 高分辨 MTK 100 mm, 1.7 μm 150 mm, 3.5 μm 186004976
HSS C18 SB 1.8 to 3.5 μm 高分辨 MTK 100 mm, 1.7 μm 150 mm, 3.5 μm 186004977
HSS Cyano 1.8 to 3.5 μm 高分辨 MTK 100 mm, 1.8 μm 150 mm, 3.5 μm 186005981
HSS PFP 1.8 to 3.5 μm 高分辨 MTK 100 mm, 1.8 μm 150 mm, 3.5 μm 186006002
*每個套裝都含有一根UPLC色譜柱和一根HPLC色譜柱，可從ACQUITY UPLC在線社區(qū)
www.waters.com/myuplc下載ACQUITY UPLC Columns Calculator用于計算方法轉(zhuǎn)移。
ACQUITY UPLC方法驗證套裝[MVK:Method Validation Kit]**
固定相 粒徑 色譜柱長度 部件編號2.1 mm ID 部件編號3.0 mm ID
CSH C18 1.7 μm 50 mm 186005571 186005573
CSH C18 1.7 μm 100 mm 186005572 186005574
CSH 苯己基1.7 μm 50 mm 186005579 186005581
CSH 苯己基1.7 μm 100 mm 186005580 186005582
CSH 氟苯基1.7 μm 50 mm 186005575 186005577
CSH 氟苯基1.7 μm 100 mm 186005576 186005578
BEH C18 1.7 μm 50 mm 186004044 186004691
BEH C18 1.7 μm 100 mm 186004045 186004692
BEH C8 1.7 μm 50 mm 186004046 186004693
BEH C8 1.7 μm 100 mm 186004047 186004694
BEH Shield RP18 1.7 μm 50 mm 186004048 186004695
BEH Shield RP18 1.7 μm 100 mm 186004049 186004696
BEH Phenyl 1.7 μm 50 mm 186004050 186004697
BEH Phenyl 1.7 μm 100 mm 186004052 186004698
BEH HILIC 1.7 μm 50 mm 186004053 186004699
BEH HILIC 1.7 μm 100 mm 186004054 186004700
BEH Amide 1.7 μm 50 mm 186004807 186004809
BEH Amide 1.7 μm 100 mm 186004808 186004810
BEH130 C18 1.7 μm 100 mm 186004896 -
BEH300 C18 1.7 μm 100 mm 186004897 -
BEH300 C4 1.7 μm 100 mm 186004899 -
寡核苷酸 C18 1.7 μm 100 mm 186004898 -
Glycan BEH Amide 1.7 μm 100 mm 186004907 -
HSS T3 1.8 μm 50 mm 186004055 186004701
HSS T3 1.8 μm 100 mm 186004056 186004702
HSS C18 1.8 μm 50 mm 186004057 186004703
HSS C18 1.8 μm 100 mm 186004058 186004704
HSS C18 SB 1.8 μm 50 mm 186004137 186004705
HSS C18 SB 1.8 μm 100 mm 186004138 186004709
HSS PFP 1.8 μm 50 mm 186005975 -
HSS PFP 1.8 μm 100 mm 186005976 -
HSS PFP 1.8 μm 50 mm 186005977 -
HSS PFP 1.8 μm 100 mm 186005978 -
HSS 氰基1.8 μm 50 mm 186005996 186005998
HSS 氰基1.8 μm 100 mm 186005997 186005999
**每個套裝包括來自3個不同批次填料的3根色譜柱。
