美國戴安 P/N: 063278Dionex ProPac IMAC Column 生物色譜柱
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產品名稱: 美國戴安 P/N: 063278Dionex ProPac IMAC Column 生物色譜柱
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簡單介紹
【簡單介紹】戴安公司生產的儀器主要有離子色譜儀、高效液相色譜儀、快速溶劑萃取儀和氨基酸直接分析儀。其中離子色譜儀憑借著雄厚的技術優勢和方法已廣泛地應用于各個領域，其市場占有率達80%。全球與離子色譜相關的文章有90%均出自戴安公司生產的離子色譜儀。
戴安公司的快速溶劑萃取儀15分鐘之內僅用15毫升溶劑既可完成對10g樣品的萃取，該技術已在環境、**、食品和聚合
美國戴安 P/N: 063278Dionex ProPac IMAC Column 生物色譜柱
的詳細介紹
Analytical Column for Immobilized Metal Affinity Chromatography
The ProPac??IMAC-10 is an analytical HPLC column packed with 10 ?m, nonporous, polymeric beads that are coated with a hydrophilic layer, then grafted with poly(IDA) chains. The poly(IDA) grafts are converted to metal-containing nanoparticles when the column is charged with metal. It is these nanoparticles that act as IMAC interaction sites for individual proteins and provide the ProPac IMAC-10 with its high resolving power.
- State-of-the-art technology for reusable columns with metal tailored specificity
- Resolve target proteins using a single column in a high resolution gradient run
- Retention control by imidazole concentration or pH gradient
- High purity separations of metal-binding proteins
- Wide range of metal-specific applications?
The ProPac IMAC-10 design maximizes the number of sterically accessible ligands for binding to proteins, ensuring that the proteins remain tightly bound during separation. It can be operated between pH 2–12 and is compatible with commonly used protein purification reagents (non-ionic detergents, reducing agents). It can be used under native or denaturing HPLC conditions?
The ProPac IMAC-10 is shipped free from any metal and ready to be charged with the metal of your choice. To avoid contact of the metal solution with your system, charge the IMAC-10 with a loading column (an empty column body you fill with your metal solution, then place between the pump and the IMAC-10 column).
| ProPac IMAC Columns?Specifications |
|---|
| Maximum Operating Pressure | <3000 psi (21 MPa) |
| Temperature Range | <60oC
Recommended?Temperature:? Controlled ambient |
| Typical Operating Conditions | <600 psi at 1.0 mL/min (4 × 250mm)
Recommended flow rate: 1.0 mL/min |
| Mobile Phase Compatibility | pH 2–12, Compatible with most reagents used in IMAC protein purifications, including EtOH, Urea, NaCl, non-ionic detergents, glycerol, acetic acid, guanidine, HCl |
| Resin Compostion | 10 ?m diameter nonporous polystyrene divinylbenzene substrate (55% crosslinking) with poly(IDA) grafts. |
| Metal Ion Capacity (?mol Cu) | ?>80 mmol Cu (4 × 250 mm analytical column) |
| Chelating Metals | Ni, Fe, Cu, others |
| Analytical Column Dimensions | 1 × 50 mm, 2 × 50 mm, 4 × 50 mm, 9 × 50 mm
2 × 250 mm, 4 × 250 mm, 9 × 250 mm, 22 × 250 mm |
| Loading Column Dimensions | 4 × 50 mm, 9 × 50 mm, 9 × 250 mm |
| Analytical Columns |
|---|
| ProPac IMAC-10 Column (1 x 50 mm) | 063617 |
| ProPac IMAC-10 Column (2 x 50 mm) | 063272 |
| ProPac IMAC-10 Column (4 x 50 mm) | 063276 |
| ProPac IMAC-10 Column (9 x 50 mm) | 063615 |
| ProPac IMAC-10 Column (2 x 250 mm) | 063274 |
| ProPac IMAC-10 Column (4 x 250 mm) | 063278 |
| ProPac IMAC-10 Column (9 x 250 mm) | 063280 |
| ProPac IMAC-10 Column (22 x 250 mm) | 063282 |
| Accessories |
|---|
| IMAC Loading Column (4 x 50 mm) | 063667 |
| IMAC Loading Column (9 x 50 mm) | 063710 |
| IMAC Loading Column (9 x 250 mm) | 063718 |
