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SP5578 美國戴安Dionex CarboPac PA10 Carbohydrate Column生物色譜柱

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產品名稱: SP5578 美國戴安Dionex CarboPac PA10 Carbohydrate Column生物色譜柱
產品型號: SP5578
產品展商: 戴安Dionex
產品文檔: 無相關文檔

簡單介紹

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SP5578 美國戴安Dionex CarboPac PA10 Carbohydrate Column生物色譜柱  的詳細介紹

CarboPac PA10 Carbohydrate Column

For Sensitive Analysis of Mono- and Disaccharides

The Thermo Scientific? CarboPac? PA10 column line has been expanded to include a new 0.4 × 250 mm capillary column. As with all CarboPac PA10 columns, the new capillary format is the ideal choice for mono- and disaccharides in foods, drugs and plants.

The CarboPac PA10 is an ideal choice for separations of mono- and disaccharides found in mammalian glycoproteins. Fucose is well resolved from the system void and from protein hydrolysate interferents. The CarboPac PA10 allows oxygen to elute after mannose, eliminating interference with monosaccharides. It is ideal for the analysis of mono- and disaccharides in foods, drugs, and plants, and separates sialic acids with the addition of sodium acetate to the eluent.

  • For the quantification of acidic, neutral, and aminomonosaccharides
  • Simple, isocratic separations
  • Superior selectivity for common monosaccharides compared to the CarboPac PA1
  • No derivatization required
  • Gradient compatible

The CarboPac PA10 is an anion-exchange column designed specifically for high-resolution separations of mono- and disaccharides. Separation is accomplished with a simple isocratic eluent, and detection is accomplished with pulsed amperometric detection (PAD). No derivatization is required.

CarboPac PA10 Column for Monosaccharide and Disaccharide Analysis  Specifications
Dimensions CarboPac PA10 Analytical Column:
4 × 250 mm, 2 × 250 mm and 0.4 × 250 mm
CarboPac PA10 Guard Column:
4 × 50 mm, 2 × 50 mm and 0.4 × 50 mm
Maximum operating pressure 3500 psi (24.5 MPa)
Temperature range 4–55 °C
Recommended operating temperature: ambient
Typical operating conditions 1900 psi at 1.0 mL/min (4 mm analytical and guard columns)
Recommended flow rate: 1.0 mL/min (4 mm), 0.25 mL/min (2 mm) or 0.010 mL/min (0.4 mm)
Mobile phase compatibility pH 0–14; up to 90% of common HPLC solvents.
Avoid anionic detergents and 100% water.
Acetate or hydroxide eluents only.
Resin composition 10-μm diameter substrate (ethylvinylbenzene 55% cross-linked with divinylbenzene) agglomerated with 460-nm MicroBead? difunctional quaternary ammonium ion (5% cross-linked).
Anion-exchange capacity Approximately:
100 μeq/column (4 × 250 mm analytical column)
25 μeq/column (2 × 250 mm analytical column)
1 μeq/column (0.4 × 250 mm analytical column)
Column construction PEEK with 10-32 threaded ferrule-style end fittings.
All components are nonmetallic.
Analytical Columns
CarboPac PA10 Analytical Column (2 x 250 mm) 057180
CarboPac PA10 Analytical Column (4 x 250 mm) 046110
CarboPac PA10 Semi-Preparative (9 X 250 mm) SP4216
Guard Columns
CarboPac PA10 Guard Column (2 x 50 mm) 057181
AminoTrap Column (2 x 50 mm) SP5578
CarboPac PA10 Guard Column (4 x 50 mm) 046115
Amino Trap Column (4 x 50 mm) 046122
Borate Trap Column 047078
Amino Trap Column (3 x 30 mm) 060146

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