Fast, Sensitive Mono- and Disaccharide Analysis
The Thermo Scientific Dionex CarboPac? PA20 column line has been expanded to include a new 0.4 × 150 mm
capillary column and a new Dionex CarboPac PA20 Fast Sialic Acid column.
As
with all CarboPac PA20 columns, the new capillary format provides
high-resolution separations of mono- and disaccharides with no need for
derivatization. The Dionex CarboPac PA20, 0.4 × 150 mm format offers the
additional advantages of higher mass sensitivity, lower sample amount
requirements, and significantly lower solvent use.
The new Dionex
CarboPac PA20 Fast Sialic Acid column is ideally suited for the fast
analysis of sialic acids in glycoprotein acid hydrolyzates. The Dionex
CarboPac PA20 Fast Sialic Acid column accelerates sialic acid
determination allowing sample analysis in less than five minutes. The
Dionex CarboPac PA20 Fast Sialic Acid column is packed with the same
hydrophobic, polymeric, pellicular anion exchange resin as the Dionex
CarboPac PA20 column, but in a 3 x 30 mm column format.
The Dionex
CarboPac PA20 is a strong anion-exchange column that provides
high-resolution separations of mono- and disaccharides with no need for
derivatization. This column provides baseline resolution between the six
monosaccharides commonly found in mammalian glycoproteins over a wide
range of eluent concentrations. The Dionex CarboPac PA20 provides
slightly different selectivity from either the Dionex CarboPac PA1 or
Dionex CarboPac PA10, making it an attractive choice for separating
difficult peak pairs.
-
The column of choice for high-sensitivity monosaccharide analyses
-
Highly efficient separations with good resolution between neighboring peaks
-
Fast analysis—less than 10 min
-
Optimized resolution of glucosamine/galactose and glucose/mannose peak pairs
-
Lower eluent consumption
-
Does not require derivatization
The
Dionex CarboPac PA20 consists of 6.5 μm-diameter nonporous beads
covered with a fine latex of functionalized Thermo Scientific Dionex
MicroBead? resin. This pellicular resin structure permits
excellent mass transfer, resulting in high-resolution chromatography and
rapid re-equilibration. The performance of each column is verified with
a Thermo Scientific Dionex MonoStandard?.
