Reversed-Phase and Weak Cation-Exchange on a Single Column
The Acclaim? Mixed-Mode WCX-1 is a novel, high-efficiency, silica-based column,
manufactured by bonding a specially designed proprietary ligand with
both hydrophobic and weak cation-exchange properties. Selectivity of
ionizable and neutral compounds can be controlled independently or
simultaneously by tuning mobile phase ionic strength, pH or organic
modifier. This column therefore can separate using multiple separation
modes: reversed phase, cation exchange, and normal phase/HILIC.
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Adjustable selectivity
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Ideal selectivity for separating basic molecules
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Selectivity complementary to RP columns
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Multimode separation mechanism: RP, Cation-exchange, Ion-exclusion and HILIC
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Capable of separating a mixture of neutral and basic compounds
The
adjustable selectivity of the Mixed-Mode WCX-1 makes it an ideal column
for secondary methods development since the selectivity is orthogonal
to C18 columns and weak anion-exchange (WAX) columns.
The Acclaim
Mixed-Mode WCX-1 columns novel column chemistry offers multiple
retention mechanisms, including RPLC, cation-exchange, ion-exclusion and
HILIC. This feature makes this column versatile in many applications
that require different selectivities.
Broad Range of Applications
Basic compounds are important in a variety of industrial applications, including pharmaceutical, chemical, consumer products, food & beverages,
and more. However, analyses of these compounds are often challenging
when using reversed-phase silica columns. The Acclaim Mixed-Mode WCX-1
provides a remedy to these difficulties: not only does it retain basic
molecules (from highly hydrophilic to highly hydrophobic), but also
separates them with symmetrical peak shapes and excellent efficiency.
The
Acclaim Mixed-Mode WCX-1 column can serve as a primary column for a
variety of basic analytes containing samples as well as a secondary
column that provides selectivity orthogonal to that of reversed-phase
columns. Thus it is an invaluable tool for HPLC method development for a
broad range of applications.
